bradford assay blank protein Best Answer bob1, 13 October 2016 - 01:05 PM You are correct, after blanking the line should pass through 0. I substracted the OD with the blank before plotting the standard curve. Unfortunately, there is a large degree of curvature over a broad range of protein concentrations (Fig. As to whether you should include 0 as a concentration in a standard curve is up to you, but I would look at where you expect your samples to … For standard, I used BSA serially diluted in dH2O, so obviously the blank is dH2O. - (Oct/05/2016 ) Hi all, I've recently done bradford assay and am so confused if I did it right. The Bradford protein assay (1) is one of several simple methods commonly used to … The Bradford assay has been used reliably for decades to quantify protein in solution. The Coomassie brilliant blue protein assay, commonly known as the Bradford assay 1, is widely used because of its rapid and convenient protocol as well as its relative sensitivity. Bradford assay which is the blank? 1).